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DNA Fluorescent dyes: Xanthene succinimidyl ester derivates have been synthesized as amine-reactive derivative able to yield stable covalently labelled biopolymers. The spectral changes associated with its neutral-anion transition allows to be used as a nucleic acid probe in a homogeneous assay. |
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Excited-state proton transfer reactions.: Our research in the last few years involved the kinetic characterization of excited-state proton transfer reactions and their effect on fluorophores’ properties. We determined the relevant rate constants of the ESPT reaction and the spectral parameters related to absorption and emission, kinetic equations and expressions for the fluorescence decay surface. For this, we made use a new global compartmental analysis approach. |
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